Environmentally Responsible Bioengineering for Spore Surface Expression of Helicobacter pylori Antigen

The development of genetic technologies and bioengineering are creating an increasing number genetically engineered microorganisms with new traits for diverse industrial applications such as vaccines, drugs pollutant degraders. However, the destiny bacterial spores released into environment long-life organisms has remained a big environmental challenge. In this study, environmentally responsible sustainable gene technology solution based on concept thymine starvation is successfully applied cloning expression Helicobacter pylori antigen Bacillus subtilis spore surface. As example, recombinant strain A1.13 been created from fusion corresponding N-terminal fragment coat protein CotB in B. entire urease subunit A (UreA) H. showed high stability surface expression. outcomes can open door developing highly safe vectored vaccines against kind pathogen contributing to reduced potential risks environment.